mesenchymal stem cells bm mscs  (ATCC)

Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: Representative cell morphology of BM-MSCs at the third passage as observed under a light microscope at 4× magnification.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Light Microscopy

Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: ( A ) Alizarin red S staining revealed the presence of calcium deposits 21 days after the induction of osteogenic medium (OM) in the presence of CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( B ) Pellet cultures of BM-MSCs were sectioned and stained with H&E, showing the morphology at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( C ) Pellet cultures of BM-MSCs were sectioned and stained with toluidine blue, showing glycosaminoglycans at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( D ) Pellet cultures of BM-MSCs were sectioned and stained with Alcian blue, showing proteoglycans at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Staining

Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: ( A ) The effects of different doses of corilagin (1, 5 and 10 µg/mL) on osteogenic differentiation potentials (Runx2, OPN) of BM-MSCs on day 21. Gene expression of differentiation potentials was assessed using real-time PCR. The bar means SD. Data is shown as mean ± SD. Statistical significance was calculated using a one-way ANOVA test and significance is represented on graphs as * p -value < 0.05. ( B ) The effects of different doses of corilagin (1, 5 and 10 µg/mL) on chondrogenic differentiation potentials (Sox9, Aggrecan) of BM-MSCs on day 21. Gene expression of differentiation potentials was assessed using real-time PCR. The bar means SD. Data is shown as mean ± SD. Statistical significance was calculated using a one-way ANOVA test and significance is represented on graphs as * p -value < 0.05.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

doi: 10.1186/s13018-025-06478-5

Figure Lengend Snippet: Interaction between DLX6-AS1 and miR-141-3p. A Predicted target sites. B Dual-luciferase reporter validation of the interaction. C Correlation analysis of serum DLX6-AS1 and miR-141-3p levels. D , E Expression of chondrogenic differentiation markers D , DLX6-AS1, and miR-141-3p E during hBMSCs chondrogenic differentiation. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

Techniques: Luciferase, Biomarker Discovery, Expressing

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

doi: 10.1186/s13018-025-06478-5

Figure Lengend Snippet: Effect of DLX6-AS1/miR-141-3p on hBMSCs functions. A qRT-PCR analysis of DLX6-AS1 and miR-141-3p after si-DLX6-AS1 treatment with or without miR-inhibition. B Proliferation level. C Apoptosis rate. D Expression of chondrogenic differentiation markers SOX9, COL2A1, and ACAN. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

Techniques: Quantitative RT-PCR, Inhibition, Expressing

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

doi: 10.1186/s13018-025-06478-5

Figure Lengend Snippet: VEGFA is a binding target of miR-141-3p. A Predicted binding sequence. B Dual-luciferase assay validation. C VEGFA expression levels in NFH (n = 96) vs DFH (n = 96) patients. D , E Correlation analyses between VEGFA and miR-141-3p or DLX6-AS1 (n = 96). Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA. hBMSCs, Human bone marrow mesenchymal stem cells. NFH, normal fracture healing; DFH, delayed fracture healing. ** P < 0.01, *** P < 0.001

Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

Techniques: Binding Assay, Sequencing, Luciferase, Biomarker Discovery, Expressing

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

doi: 10.1186/s13018-025-06478-5

Figure Lengend Snippet: Functional role of the miR-141-3p/VEGFA axis in hBMSCs. A miR-141-3p and VEGFA abundance after transfection. B Proliferation assessed. C Apoptosis analysis. D Expression of chondrogenic differentiation markers after indicated treatments. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

Techniques: Functional Assay, Transfection, Expressing